Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody

产品详情

产品名称

Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody

产品货号

YM-A03878

规格

50μL;100μL

种属反应

Human

实验应用

WB,ELISA

分子量

47,22kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270

特异性

Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4/5 p20 protein resulting from cleavage adjacent to D270/D311.

基因名称

CASP4

蛋白名称

Caspase4

别名

CASP4;ICH2;Caspase-4;CASP-4;ICE;rel;-II;Protease ICH-2;Protease TX;CASP5;ICH3;Caspase-5;CASP-5;ICE;rel;-III;Protease ICH-3;Protease TY

基因ID-1

837

SwissProt

P49662;P51878

背景

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

细胞定位

Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630). .