Cleaved-COL4A3 (L1425) Polyclonal Antibody

产品详情

产品名称

Cleaved-COL4A3 (L1425) Polyclonal Antibody

产品货号

YM-A03899

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,ELISA

分子量

140kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Collagen IV alpha3. AA range:1376-1425

特异性

Cleaved-COL4A3 (L1425) Polyclonal Antibody detects endogenous levels of fragment of activated COL4A3 protein resulting from cleavage adjacent to L1425.

基因名称

COL4A3

蛋白名称

Collagen alpha-3(IV) chain

别名

COL4A3;Collagen alpha-3;IV;chain;Goodpasture antigen

基因ID-1

1285

SwissProt

Q01955

背景

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal r

细胞定位

Secreted, extracellular space, extracellular matrix, basement membrane. Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL). .