His-tag Polyclonal Antibody

产品详情

产品名称

His-tag Polyclonal Antibody

产品货号

YM-A04055

规格

50μL;100μL

种属反应

Species independent

实验应用

WB,ELISA

分子量

15kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:1000-1:3000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

6X His synthetic peptide conjugated to KLH.

特异性

His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

基因名称

His-Tag

蛋白名称

His Tag

别名

6 His epitope tag;Hexa His tag;HHHHHH epitope tag;HHHHHH tag;His tag

基因ID-1

SwissProt

背景

The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

细胞定位