Ref-1 (Acetyl Lys7) Polyclonal Antibody

产品详情

产品名称

Ref-1 (Acetyl Lys7) Polyclonal Antibody

产品货号

YM-A04126

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,IHC

分子量

35kD

宿主

Rabbit

同种型

IgG

修饰

Acetyl

推荐稀释比

WB 1:500-2000;IHC 1:50-300

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7. AA range:1-50

特异性

Acetyl-Ref-1 (K7) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K7.

基因名称

APEX1

蛋白名称

DNA-(apurinic or apyrimidinic site) lyase

别名

APEX1;APE;APE1;APEX;APX;HAP1;REF1;DNA-;apurinic or apyrimidinic site;lyase;APEX nuclease;APEN;Apurinic-apyrimidinic endonuclease 1;AP endonuclease 1;APE-1;REF-1;Redox factor-1

基因ID-1

328

SwissProt

P27695

背景

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008],

细胞定位

Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. .; [DNA-(apurinic or apyrimidinic site) endonuclease, mitochondrial]: Mitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress. .