Podocalyxin-like 1 Monoclonal Antibody

产品详情

产品名称

Podocalyxin-like 1 Monoclonal Antibody

产品货号

YM-A04673

规格

50μL;100μL

种属反应

Human

实验应用

WB,IHC,IF,Flow Cyt,ELISA

分子量

宿主

Mouse

同种型

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

Affinity purification

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

克降性

Monoclonal

克隆号

免疫原

Purified recombinant fragment of human Podocalyxin-like 1 expressed in E. Coli.

特异性

Podocalyxin-like 1 Monoclonal Antibody detects endogenous levels of Podocalyxin-like 1 protein.

基因名称

PODXL

蛋白名称

Podocalyxin

别名

PODXL;PCLP;PCLP1;Podocalyxin;GCTM-2 antigen;Gp200;Podocalyxin-like protein 1;PC;PCLP-1

基因ID-1

5420

SwissProt

O00592

背景

podocalyxin like(PODXL) Homo sapiens This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008],

细胞定位

Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus . Membrane raft . Membrane ; Single-pass type I membrane protein . In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). .