MBP-Tag Polyclonal Antibody

产品详情

产品名称

MBP-Tag Polyclonal Antibody

产品货号

YM-A11741

规格

50μL;100μL

种属反应

Species independent

实验应用

WB

分子量

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:5000

组成

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

克降性

Polyclonal

克隆号

免疫原

Recombinant Protein of MBP-Tag

特异性

The antibody detects MBP & MBP tag fusion proteins.

基因名称

蛋白名称

别名

YM3209

基因ID-1

SwissProt

背景

Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.

细胞定位