GAL4 Activation Domain Polyclonal Antibody

产品详情

产品名称

GAL4 Activation Domain Polyclonal Antibody

产品货号

YM-A11778

规格

50μL;100μL

种属反应

实验应用

WB

分子量

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:10000-50000

组成

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

克降性

Polyclonal

克隆号

免疫原

Recombinant Protein of GAL4 Activation Domain

特异性

The antibody detects endogenous GAL4 Activation Domain protein.

基因名称

蛋白名称

别名

YM3410

基因ID-1

SwissProt

背景

The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.

细胞定位