EYFP Polyclonal Antibody

产品详情

产品名称

EYFP Polyclonal Antibody

产品货号

YM-A11787

规格

50μL;100μL

种属反应

Species independent

实验应用

WB

分子量

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:3000-5000

组成

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

克降性

Polyclonal

克隆号

免疫原

Recombinant Protein of EYFP

特异性

The antibody detects EYFP YFP CFP morange tag fusion proteins. It is also reactive against GFP tag weakly

基因名称

蛋白名称

别名

YM3229

基因ID-1

SwissProt

背景

Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.

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