Chk1 (phospho Ser280) Polyclonal Antibody

产品详情

产品名称

Chk1 (phospho Ser280) Polyclonal Antibody

产品货号

YM-A15010

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,ELISA

分子量

54kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser280. AA range:251-300

特异性

Phospho-Chk1 (S280) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S280.

基因名称

CHEK1

蛋白名称

Serine/threonine-protein kinase Chk1

别名

CHEK1;CHK1;Serine/threonine-protein kinase Chk1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1

基因ID-1

1111

SwissProt

O14757

背景

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],

细胞定位

Nucleus . Chromosome . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). .