Chk2 (phospho Thr68) Polyclonal Antibody

产品详情

产品名称

Chk2 (phospho Thr68) Polyclonal Antibody

产品货号

YM-A15014

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IF,WB,IHC,ELISA

分子量

61kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;Not yet tested in other applications;

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68. AA range:35-84

特异性

Phospho-Chk2 (T68) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.

基因名称

CHEK2

蛋白名称

Serine/threonine-protein kinase Chk2

别名

CHEK2;CDS1;CHK2;RAD53;Serine/threonine-protein kinase Chk2;CHK2 checkpoint homolog;Cds1 homolog;Hucds1;hCds1;Checkpoint kinase 2

基因ID-1

11200

SwissProt

O96017

背景

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati

细胞定位

[Isoform 2]: Nucleus. Isoform 10 is present throughout the cell.; [Isoform 4]: Nucleus.; [Isoform 7]: Nucleus.; [Isoform 9]: Nucleus.; [Isoform 12]: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Recruited into PML bodies together with TP53.