Cyclin E1 (phospho Thr395) Polyclonal Antibody

产品详情

产品名称

Cyclin E1 (phospho Thr395) Polyclonal Antibody

产品货号

YM-A15030

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,IHC,IF,ELISA

分子量

48kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr395. AA range:361-410

特异性

Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395.

基因名称

CCNE1

蛋白名称

G1/S-specific cyclin-E1

别名

CCNE1;CCNE;G1/S-specific cyclin-E1

基因ID-1

898

SwissProt

P24864

背景

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

细胞定位

Nucleus .