PKC ζ (phospho Thr410) Polyclonal Antibody

产品详情

产品名称

PKC ζ (phospho Thr410) Polyclonal Antibody

产品货号

YM-A15178

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IHC,IF,ELISA

分子量

70kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr410. AA range:376-425

特异性

Phospho-PKC ζ (T410) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T410.

基因名称

PRKCZ

蛋白名称

Protein kinase C zeta type

别名

PRKCZ;PKC2;Protein kinase C zeta type;nPKC-zeta

基因ID-1

5590

SwissProt

Q05513

背景

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

细胞定位

Cytoplasm . Endosome . Cell junction . Membrane ; Peripheral membrane protein . In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes (PubMed:9566925). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083). Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651). Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity). .; [Isoform 2]: Cytoplasm .