PKC θ (phospho Ser676) Polyclonal Antibody

产品详情

产品名称

PKC θ (phospho Ser676) Polyclonal Antibody

产品货号

YM-A15180

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IHC,IF,ELISA

分子量

82kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Ser676. AA range:643-692

特异性

Phospho-PKC θ (S676) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at S676.

基因名称

PRKCQ

蛋白名称

Protein kinase C theta type

别名

PRKCQ;PRKCT;Protein kinase C theta type;nPKC-theta

基因ID-1

5588

SwissProt

Q04759

背景

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],

细胞定位

Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.