PTEN (phospho Ser380) Polyclonal Antibody

产品详情

产品名称

PTEN (phospho Ser380) Polyclonal Antibody

产品货号

YM-A15187

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IHC,IF,ELISA

分子量

55kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human PTEN around the phosphorylation site of Ser380. AA range:366-395

特异性

Phospho-PTEN (S380) Polyclonal Antibody detects endogenous levels of PTEN protein only when phosphorylated at S380.

基因名称

PTEN

蛋白名称

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

别名

PTEN;MMAC1;TEP1;Phosphatidylinositol 3;4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog

基因ID-1

5729

SwissProt

P60484

背景

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate ener

细胞定位

Cytoplasm . Nucleus . Nucleus, PML body . Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620). XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982). .; [Isoform alpha]: Secreted . May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif.