Stat3 (phospho Tyr705) Polyclonal Antibody

产品详情

产品名称

Stat3 (phospho Tyr705) Polyclonal Antibody

产品货号

YM-A15200

规格

50μL;100μL

种属反应

Human,Mouse,Rat,Pig(Test by out customer)

实验应用

IF,WB,IHC,IP,ELISA

分子量

88kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:20000;Not yet tested in other applications;

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Tyr705. AA range:672-721

特异性

Phospho-Stat3 (Y705) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at Y705.

基因名称

STAT3

蛋白名称

Signal transducer and activator of transcription 3

别名

STAT3;APRF;Signal transducer and activator of transcription 3;Acute-phase response factor

基因ID-1

6774

SwissProt

P40763

背景

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper

细胞定位

Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.