Raf-1 (phospho Ser43) Polyclonal Antibody

产品详情

产品名称

Raf-1 (phospho Ser43) Polyclonal Antibody

产品货号

YM-A15302

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IF,ELISA,IHC

分子量

73kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser43. AA range:11-60

特异性

Phospho-Raf-1 (S43) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S43.

基因名称

RAF1

蛋白名称

RAF proto-oncogene serine/threonine-protein kinase

别名

RAF1;RAF;RAF proto-oncogene serine/threonine-protein kinase;Proto-oncogene c-RAF;cRaf;Raf-1

基因ID-1

5894

SwissProt

P04049

背景

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

细胞定位

Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.