ERK 8 (phospho Thr175/Y177) Polyclonal Antibody

产品详情

产品名称

ERK 8 (phospho Thr175/Y177) Polyclonal Antibody

产品货号

YM-A15306

规格

50μL;100μL

种属反应

Human,Mouse

实验应用

WB,IHC,IF,ELISA

分子量

60kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190

特异性

Phospho-ERK 8 (T175/Y177) Polyclonal Antibody detects endogenous levels of ERK 8 protein only when phosphorylated at T175/Y177.

基因名称

MAPK15

蛋白名称

Mitogen-activated protein kinase 15

别名

MAPK15;ERK7;ERK8;Mitogen-activated protein kinase 15;MAP kinase 15;MAPK 15;Extracellular signal-regulated kinase 7;ERK-7;Extracellular signal-regulated kinase 8;ERK-8

基因ID-1

225689

SwissProt

Q8TD08

背景

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

细胞定位

Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). .