c-Src (phospho Tyr216) Polyclonal Antibody

产品详情

产品名称

c-Src (phospho Tyr216) Polyclonal Antibody

产品货号

YM-A15400

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IHC,IF,ELISA

分子量

50kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr216. AA range:181-230

特异性

Phospho-c-Src (Y216) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y216.

基因名称

SRC

蛋白名称

Proto-oncogene tyrosine-protein kinase Src

别名

SRC;SRC1;Proto-oncogene tyrosine-protein kinase Src;Proto-oncogene c-Src;pp60c-src;p60-Src

基因ID-1

6714

SwissProt

P12931

背景

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

细胞定位

Cell membrane ; Lipid-anchor . Mitochondrion inner membrane . Nucleus . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Cell junction, focal adhesion . Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). .