c-Abl (phospho Tyr245) Polyclonal Antibody

产品详情

产品名称

c-Abl (phospho Tyr245) Polyclonal Antibody

产品货号

YM-A15482

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IF,ELISA

分子量

125kD(200kD BCR-ABL complex）

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human c-Abl around the phosphorylation site of Tyr245. AA range:196-245

特异性

Phospho-c-Abl (Y245) Polyclonal Antibody detects endogenous levels of c-Abl protein only when phosphorylated at Y245.

基因名称

ABL1

蛋白名称

Tyrosine-protein kinase ABL1

别名

ABL1;ABL;JTK7;Tyrosine-protein kinase ABL1;Abelson murine leukemia viral oncogene homolog 1;Abelson tyrosine-protein kinase 1;Proto-oncogene c-Abl;p150

基因ID-1

25

SwissProt

P00519

背景

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr

细胞定位

Cytoplasm, cytoskeleton. Nucleus. Mitochondrion . Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity). .; [Isoform IB]: Nucleus membrane; Lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear.