Smad3 (phospho Ser425) Polyclonal Antibody

产品详情

产品名称

Smad3 (phospho Ser425) Polyclonal Antibody

产品货号

YM-A15533

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IF,WB,IHC,ELISA

分子量

58kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications;

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425

特异性

Phospho-Smad3 (S425) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S425.

基因名称

SMAD3

蛋白名称

Mothers against decapentaplegic homolog 3

别名

SMAD3;MADH3;Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3

基因ID-1

4088

SwissProt

P84022

背景

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

细胞定位

Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .