PAKα/β/γ (phospho Ser144/141/139) Polyclonal Antibody

产品详情

产品名称

PAKα/β/γ (phospho Ser144/141/139) Polyclonal Antibody

产品货号

YM-A15615

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,IHC,IF,ELISA

分子量

65kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human PAK1/2/3 around the phosphorylation site of Ser144/141/139. AA range:111-160

特异性

Phospho-PAKα/β/γ (S144/141/139) Polyclonal Antibody detects endogenous levels of PAKα/β/γ protein only when phosphorylated at S144/141/139.

基因名称

PAK1/PAK2/PAK3

蛋白名称

Serine/threonine-protein kinase PAK 1/Serine/threonine-protein kinase PAK 2/Serine/threonine-protein kinase PAK 3

别名

PAK1;Serine/threonine-protein kinase PAK 1;Alpha-PAK;p21-activated kinase 1;PAK-1;p65-PAK;PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58;PAK3;OPHN3;Serine/threonine-p

基因ID-1

5058;5062;5063

SwissProt

Q13153;Q13177;O75914

背景

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],

细胞定位

Cytoplasm . Cell junction, focal adhesion . Cell projection, lamellipodium . Cell membrane . Cell projection, ruffle membrane . Cell projection, invadopodium . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667). Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601). Localization of the active, but not inactive, protein to the adhesions and edge of lamellipodia is mediated by interaction with GIT1 (PubMed:11896197). .