AMPKβ1 (phospho Ser182) Polyclonal Antibody

产品详情

产品名称

AMPKβ1 (phospho Ser182) Polyclonal Antibody

产品货号

YM-A15662

规格

50μL;100μL

种属反应

Human,Mouse,Rat,Monkey

实验应用

WB,IHC,IF,ELISA

分子量

33kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human AMPK beta1 around the phosphorylation site of Ser181. AA range:147-196

特异性

Phospho-AMPKβ1 (S182) Polyclonal Antibody detects endogenous levels of AMPKβ1 protein only when phosphorylated at S182.

基因名称

PRKAB1

蛋白名称

5'-AMP-activated protein kinase subunit beta-1

别名

PRKAB1;AMPK;5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb

基因ID-1

5564

SwissProt

Q9Y478

背景

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided

细胞定位

nucleus,nucleoplasm,cytosol,nucleotide-activated protein kinase complex,