Splicing factor 1 (phospho Ser82) Polyclonal Antibody

产品详情

产品名称

Splicing factor 1 (phospho Ser82) Polyclonal Antibody

产品货号

YM-A15841

规格

50μL;100μL

种属反应

Human,Mouse,Monkey

实验应用

WB,IHC,IF,ELISA

分子量

68kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human SF1 around the phosphorylation site of Ser82. AA range:48-97

特异性

Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.

基因名称

SF1

蛋白名称

Splicing factor 1

别名

SF1;ZFM1;ZNF162;Splicing factor 1;Mammalian branch point-binding protein;BBP;mBBP;Transcription factor ZFM1;Zinc finger gene in MEN1 locus;Zinc finger protein 162

基因ID-1

7536

SwissProt

Q15637

背景

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016],

细胞定位

Nucleus.