Rac GAP1 (phospho Ser387) Polyclonal Antibody

产品详情

产品名称

Rac GAP1 (phospho Ser387) Polyclonal Antibody

产品货号

YM-A15880

规格

50μL;100μL

种属反应

Human,Mouse,Rat,Monkey

实验应用

WB,IHC,IF,ELISA

分子量

72kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human GTPase Activating Protein around the phosphorylation site of Ser387. AA range:353-402

特异性

Phospho-Rac GAP1 (S387) Polyclonal Antibody detects endogenous levels of Rac GAP1 protein only when phosphorylated at S387.

基因名称

RACGAP1

蛋白名称

Rac GTPase-activating protein 1

别名

RACGAP1;KIAA1478;MGCRACGAP;Rac GTPase-activating protein 1;Male germ cell RacGap;MgcRacGAP;Protein CYK4 homolg;CYK4;HsCYK-4

基因ID-1

29127

SwissProt

Q9H0H5

背景

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016],

细胞定位

Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle . Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody, Midbody ring . Cell membrane; Peripheral membrane protein; Cytoplasmic side. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody ring, also called Flemming body. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. .