ACAP1 (phospho Ser554) Polyclonal Antibody

产品详情

产品名称

ACAP1 (phospho Ser554) Polyclonal Antibody

产品货号

YM-A15962

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,IHC,IF,ELISA

分子量

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:500-2000;WB 1:500-2000;IHC 1:100-1:300;ELISA 1:10000-40000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Centaurin-beta1 around the phosphorylation site of Ser554. AA range:520-569

特异性

Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554.

基因名称

ACAP1

蛋白名称

Arf-GAP with coiled-coil ANK repeat and PH domain-containing protein 1

别名

ACAP1;CENTB1;KIAA0050;Arf-GAP with coiled-coil;ANK repeat and PH domain-containing protein 1;Centaurin-beta-1;Cnt-b1

基因ID-1

9744

SwissProt

Q15027

背景

domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes.,PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 BAR domain.,similarity:Contains 1 PH domain.,similarity:Contains 3 ANK repeats.,subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1.,tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.,

细胞定位

Recycling endosome membrane ; Peripheral membrane protein ; Cytoplasmic side .