CENP-A (phospho Ser7) Polyclonal Antibody

产品详情

产品名称

CENP-A (phospho Ser7) Polyclonal Antibody

产品货号

YM-A16117

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

IF,ELISA

分子量

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications;

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human Centromeric Protein A around the phosphorylation site of Ser7. AA range:1-50

特异性

Phospho-CENP-A (S7) Polyclonal Antibody detects endogenous levels of CENP-A protein only when phosphorylated at S7.

基因名称

CENPA

蛋白名称

Histone H3-like centromeric protein A

别名

CENPA;Histone H3-like centromeric protein A;Centromere autoantigen A;Centromere protein A;CENP-A

基因ID-1

1058

SwissProt

P49450

背景

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015],

细胞定位

Nucleus . Chromosome, centromere, kinetochore . Chromosome, centromere . Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658). .