Rpb1 CTD (Phospho Thr4) rabbit pAb

产品详情

产品名称

Rpb1 CTD (Phospho Thr4) rabbit pAb

产品货号

YM-A16394

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB

分子量

250kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:1000-2000

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

Synthesized phosho peptide around human Rpb1 CTD (Thr4)

特异性

This antibody detects endogenous levels of  Human Mouse Rat POLR2A carboxy-terminal domain (CTD) heptapeptide repeat YSPTSPS (phospho-Thr4)

基因名称

POLR2A POLR2

蛋白名称

Rpb1 CTD (Thr4)

别名

DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1;

基因ID-1

5430

SwissProt

P24928

背景

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008],

细胞定位

Nucleus . Cytoplasm . Chromosome . Hypophosphorylated form is mainly found in the cytoplasm, while the hyperphosphorylated and active form is nuclear (PubMed:26566685). Co-localizes with kinase SRPK2 and helicase DDX23 at chromatin loci where unscheduled R-loops form (PubMed:28076779). .