Chk2 (Phospho Thr432) rabbit pAb

产品详情

产品名称

Chk2 (Phospho Thr432) rabbit pAb

产品货号

YM-A16475

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

WB,ELISA

分子量

60kD

宿主

Rabbit

同种型

IgG

修饰

Phospho

推荐稀释比

WB 1:1000-2000;ELISA 1:5000-20000

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

Synthesized peptide derived from human Chk2 (Phospho Thr432)

特异性

This antibody detects endogenous levels of Human,Mouse,Rat Chk2 (Phospho Thr432)

基因名称

CHEK2 CDS1 CHK2 RAD53

蛋白名称

Chk2 (Phospho Thr432)

别名

Serine/threonine-protein kinase Chk2;CHK2 checkpoint homolog;Cds1 homolog;Hucds1;hCds1;Checkpoint kinase 2;

基因ID-1

11200

SwissProt

O96017

背景

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.,disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].,disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].,enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.,function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.,similarity:Contains 1 FHA domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Isoform 10 is present throughout the cell.,tissue specificity:High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.,

细胞定位

[Isoform 2]: Nucleus. Isoform 10 is present throughout the cell.; [Isoform 4]: Nucleus.; [Isoform 7]: Nucleus.; [Isoform 9]: Nucleus.; [Isoform 12]: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Recruited into PML bodies together with TP53.