Phospho Akt (S473) mouse Monoclonal Antibody(7F9)

产品详情

产品名称

Phospho Akt (S473) mouse Monoclonal Antibody(7F9)

产品货号

YM-A16763

规格

50μL;100μL

种属反应

Human

实验应用

WB,IHC,IF

分子量

56kD

宿主

Mouse

同种型

修饰

Phospho

推荐稀释比

WB 1:1000-2000;IHC 1:100-200;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Monoclonal

克隆号

7F9

免疫原

Synthetic Peptide of Phospho-Akt (S473) at AA range of 410-490

特异性

Phospho-Akt (S473) protein detects endogenous levels of AKT1

基因名称

AKT1/AKT2/AKT3

蛋白名称

AKT1/AKT2/AKT3

别名

AKT1

基因ID-1

207

SwissProt

P31749

背景

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

细胞定位

Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529). .