EID-1 Polyclonal Antibody

产品详情

产品名称

EID-1 Polyclonal Antibody

产品货号

YM-A18061

规格

50μL;100μL

种属反应

Human,Mouse

实验应用

WB,IHC,IF,ELISA

分子量

21kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human EID1. AA range:71-120

特异性

EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.

基因名称

EID1

蛋白名称

EP300-interacting inhibitor of differentiation 1

别名

EID1;C15orf3;CRI1;RBP21;PNAS-22;PTD014;EP300-interacting inhibitor of differentiation 1;21 kDa pRb-associated protein;CREBBP/EP300 inhibitory protein 1;E1A-like inhibitor of differentiation 1;EID-1

基因ID-1

23741

SwissProt

Q9Y6B2

背景

developmental stage:Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).,function:Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.,induction:Down-regulated in differentiating U937 leukemia cells.,miscellaneous:Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.,PTM:Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.,subcellular location:May shuttle between nucleus and cytoplasm.,subunit:Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.,tissue specificity:Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.,

细胞定位

Nucleus . Cytoplasm . May shuttle between nucleus and cytoplasm. .