GRF-1 Polyclonal Antibody

产品详情

产品名称

GRF-1 Polyclonal Antibody

产品货号

YM-A18532

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IHC,IF,ELISA

分子量

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human GRF-1. AA range:1071-1120

特异性

GRF-1 Polyclonal Antibody detects endogenous levels of GRF-1 protein.

基因名称

ARHGAP35

蛋白名称

Rho GTPase-activating protein 35

别名

ARHGAP35;GRF1;GRLF1;KIAA1722;Rho GTPase-activating protein 35;Glucocorticoid receptor DNA-binding factor 1;Glucocorticoid receptor repression factor 1;GRF-1;Rho GAP p190A;p190-A

基因ID-1

2909

SwissProt

Q9NRY4

背景

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008],

细胞定位

Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm . Nucleus . Cell membrane . In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin. .