MEK Kinase-3 Polyclonal Antibody

产品详情

产品名称

MEK Kinase-3 Polyclonal Antibody

产品货号

YM-A19055

规格

50μL;100μL

种属反应

Human,Mouse

实验应用

WB,IHC,IF,ELISA

分子量

71kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150

特异性

MEK Kinase-3 Polyclonal Antibody detects endogenous levels of MEK Kinase-3 protein.

基因名称

MAP3K3

蛋白名称

Mitogen-activated protein kinase kinase kinase 3

别名

MAP3K3;MAPKKK3;MEKK3;Mitogen-activated protein kinase kinase kinase 3;MAPK/ERK kinase kinase 3;MEK kinase 3;MEKK 3

基因ID-1

4215

SwissProt

Q99759

背景

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

细胞定位

cytoplasm,cytosol,