p14ARF Polyclonal Antibody

产品详情

产品名称

p14ARF Polyclonal Antibody

产品货号

YM-A19743

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,IHC,IF,ELISA

分子量

18kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human p14 ARF. AA range:71-120

特异性

p14 Polyclonal Antibody detects endogenous levels of p14 protein.

基因名称

CDKN2A

蛋白名称

Cyclin-dependent kinase inhibitor 2A isoform 4

别名

CDKN2A;CDKN2;MLM;Cyclin-dependent kinase inhibitor 2A;isoform 4;p14ARF;p19ARF

基因ID-1

1029

SwissProt

Q8N726

背景

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by CDKN2A, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. CDKN2A is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

细胞定位

Nucleus, nucleolus . Nucleus, nucleoplasm .; [Isoform smARF]: Mitochondrion .