PCNA Polyclonal Antibody

产品详情

产品名称

PCNA Polyclonal Antibody

产品货号

YM-A19820

规格

50μL;100μL

种属反应

Human,Mouse,Rat,Monkey,Rabbit

实验应用

WB,IHC,IF,ELISA

分子量

30-33kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-2000;IHC 1:100-500;IF/ICC1:100-500;ELISA 1:5000-20000

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

特异性

PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.

基因名称

PCNA

蛋白名称

Proliferating cell nuclear antigen

别名

PCNA;Proliferating cell nuclear antigen;PCNA;Cyclin

基因ID-1

5111

SwissProt

P12004

背景

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

细胞定位

Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .