Rad17 Polyclonal Antibody

产品详情

产品名称

Rad17 Polyclonal Antibody

产品货号

YM-A20111

规格

50μL;100μL

种属反应

Human,Mouse

实验应用

WB,IHC,IF,ELISA

分子量

86kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from human RAD17. AA range:621-670

特异性

Rad17 Polyclonal Antibody detects endogenous levels of Rad17 protein.

基因名称

RAD17

蛋白名称

Cell cycle checkpoint protein RAD17

别名

RAD17;R24L;Cell cycle checkpoint protein RAD17;hRad17;RF-C/activator 1 homolog

基因ID-1

5884

SwissProt

O75943

背景

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

细胞定位

Nucleus . Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.