HPA1 Polyclonal Antibody

产品详情

产品名称

HPA1 Polyclonal Antibody

产品货号

YM-A21327

规格

50μL;100μL

种属反应

Human,Rat,Mouse,

实验应用

WB,IHC,IF,ELISA

分子量

62kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human HPSE. AA range:241-290

特异性

HPA1 Polyclonal Antibody detects endogenous levels of HPA1 protein.

基因名称

HPSE

蛋白名称

Heparanase

别名

HPSE;HEP;HPA;HPA1;HPR1;HPSE1;HSE1;Heparanase;Endo-glucoronidase;Heparanase-1;Hpa1

基因ID-1

10855

SwissProt

Q9Y251

背景

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

细胞定位

Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus. Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity). .