Amyloid-β Polyclonal Antibody

产品详情

产品名称

Amyloid-β Polyclonal Antibody

产品货号

YM-A21658

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IF,WB,IHC,ELISA

分子量

87kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

Synthesized peptide derived from Amyloid-β at AA range: 221-270

特异性

Amyloid-β Polyclonal Antibody detects endogenous levels of Amyloid-β

基因名称

APP A4 AD1

蛋白名称

Amyloid beta A4 protein, Amyloid-β, Aβ

别名

amyloid beta;A4;precursor protein

基因ID-1

351

SwissProt

P05067

背景

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014],

细胞定位

Cell membrane ; Single-pass type I membrane protein . Membrane ; Single-pass type I membrane protein . Perikaryon . Cell projection, growth cone . Membrane, clathrin-coated pit . Early endosome . Cytoplasmic vesicle . Cell surface protein that rapidly becomes internalized via clathrin-coated pits. Only a minor proportion is present at the cell membrane; most of the protein is present in intracellular vesicles (PubMed:20580937). During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body (PubMed:10341243). Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. Associates with GPC1 in perinuclear compartments. Colocalizes with SORL1 in a vesicular pattern in cytoplasm and perinuclear regions. .; [C83]: Endoplasmic reticulum . Golgi apparatus . Early endosome .; [C99]: Early endosome .; [Soluble APP-beta]: Secreted .; [Amyloid-beta protein 42]: Cell surface. Associates with FPR2 at the cell surface and the complex is then rapidly internalized. .; [Gamma-secretase C-terminal fragment 59]: Nucleus . Cytoplasm . Located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65) (PubMed:11544248). In dopaminergic neurons, the phosphorylated Thr-743 form is localized to the nucleus (By similarity). .