Shh Polyclonal Antibody

产品详情

产品名称

Shh Polyclonal Antibody

产品货号

YM-A21992

规格

50μL;100μL

种属反应

Human,Mouse,Rat

实验应用

IHC,IF,WB

分子量

40kD

宿主

Rabbit

同种型

IgG

修饰

Unmodified

推荐稀释比

IHC 1:50-200;WB 1:500-2000;IF 1:50-200

组成

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化工艺

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

储存

-15°C to -25°C/1 year(Do not lower than -25°C)

浓度

1 mg/ml

克降性

Polyclonal

克隆号

免疫原

Synthesized peptide derived from human Shh

特异性

This antibody detects endogenous levels of human Shh

基因名称

SHH

蛋白名称

Shh

别名

Sonic hedgehog protein;SHH;HHG-1;[Cleaved into: Sonic hedgehog protein N-product;Sonic hedgehog protein C-product]

基因ID-1

6469

SwissProt

Q15465

背景

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a d

细胞定位

Endoplasmic reticulum membrane . Golgi apparatus membrane . Co-localizes with HHAT in the ER and Golgi membrane. .; [Sonic hedgehog protein N-product]: Cell membrane ; Lipid-anchor . The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (PubMed:24522195). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides (PubMed:26875496, PubMed:24522195). .